Identification of the Dominant Genotypes of Phytophthora infestans in Canada Using Real-Time PCR with ASO-PCR Assays.
Identifieur interne : 000C04 ( Main/Exploration ); précédent : 000C03; suivant : 000C05Identification of the Dominant Genotypes of Phytophthora infestans in Canada Using Real-Time PCR with ASO-PCR Assays.
Auteurs : Marie-Claude Gagnon [Canada] ; Lawrence Kawchuk [Canada] ; D Mathieu Tremblay [Canada] ; Odile Carisse [Canada] ; Giovanna Danies [États-Unis] ; William E. Fry [États-Unis] ; C André Lévesque [Canada] ; Guillaume J. Bilodeau [Canada]Source :
- Plant disease [ 0191-2917 ] ; 2016.
Abstract
Phytophthora infestans, a pathogenic oomycete that is the causal agent of potato and tomato late blight, has devastating effects worldwide. The genetic composition of P. infestans populations in Canada has changed considerably over the last few years, with the appearance of several new genotypes showing different mating types and sensitivity to the fungicide metalaxyl. Genetic markers allowing for a rapid assessment of genotypes from small amounts of biological material would be beneficial for the early detection and control of this pathogen throughout Canada. Mining of the P. infestans genome revealed several regions containing single-nucleotide polymorphisms (SNP) within both nuclear genes and flanking sequences of microsatellite loci. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) assays were developed from 14 of the 50 SNP found by sequencing. Nine optimized ASO-PCR assays were validated using a blind test comprising P. infestans and other Phytophthora spp. The assays revealed diagnostic profiles unique to each of the five dominant genotypes present in Canada. The markers developed in this study can be used with environmental samples such as infected leaves, and will contribute to the genomic toolbox available to assess the genetic diversity of P. infestans at the intraspecific level. For late blight management, early warning about P. infestans genotypes present in potato and tomato fields will help growers select the most appropriate fungicides and application strategies.
DOI: 10.1094/PDIS-07-15-0763-RE
PubMed: 30686184
Affiliations:
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Bilodeau</name><affiliation wicri:level="3"><nlm:affiliation>CFIA, Ottawa.</nlm:affiliation><country>Canada</country><placeName><settlement type="city">Ottawa</settlement><region type="state">Ontario</region></placeName><wicri:orgArea>CFIA</wicri:orgArea></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora infestans, a pathogenic oomycete that is the causal agent of potato and tomato late blight, has devastating effects worldwide. The genetic composition of P. infestans populations in Canada has changed considerably over the last few years, with the appearance of several new genotypes showing different mating types and sensitivity to the fungicide metalaxyl. Genetic markers allowing for a rapid assessment of genotypes from small amounts of biological material would be beneficial for the early detection and control of this pathogen throughout Canada. Mining of the P. infestans genome revealed several regions containing single-nucleotide polymorphisms (SNP) within both nuclear genes and flanking sequences of microsatellite loci. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) assays were developed from 14 of the 50 SNP found by sequencing. Nine optimized ASO-PCR assays were validated using a blind test comprising P. infestans and other Phytophthora spp. The assays revealed diagnostic profiles unique to each of the five dominant genotypes present in Canada. The markers developed in this study can be used with environmental samples such as infected leaves, and will contribute to the genomic toolbox available to assess the genetic diversity of P. infestans at the intraspecific level. For late blight management, early warning about P. infestans genotypes present in potato and tomato fields will help growers select the most appropriate fungicides and application strategies.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30686184</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>100</Volume><Issue>7</Issue><PubDate><Year>2016</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>Identification of the Dominant Genotypes of Phytophthora infestans in Canada Using Real-Time PCR with ASO-PCR Assays.</ArticleTitle><Pagination><MedlinePgn>1482-1491</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-07-15-0763-RE</ELocationID><Abstract><AbstractText>Phytophthora infestans, a pathogenic oomycete that is the causal agent of potato and tomato late blight, has devastating effects worldwide. The genetic composition of P. infestans populations in Canada has changed considerably over the last few years, with the appearance of several new genotypes showing different mating types and sensitivity to the fungicide metalaxyl. Genetic markers allowing for a rapid assessment of genotypes from small amounts of biological material would be beneficial for the early detection and control of this pathogen throughout Canada. Mining of the P. infestans genome revealed several regions containing single-nucleotide polymorphisms (SNP) within both nuclear genes and flanking sequences of microsatellite loci. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) assays were developed from 14 of the 50 SNP found by sequencing. Nine optimized ASO-PCR assays were validated using a blind test comprising P. infestans and other Phytophthora spp. The assays revealed diagnostic profiles unique to each of the five dominant genotypes present in Canada. The markers developed in this study can be used with environmental samples such as infected leaves, and will contribute to the genomic toolbox available to assess the genetic diversity of P. infestans at the intraspecific level. For late blight management, early warning about P. infestans genotypes present in potato and tomato fields will help growers select the most appropriate fungicides and application strategies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gagnon</LastName><ForeName>Marie-Claude</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Canadian Food Inspection Agency (CFIA), Ottawa, ON, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kawchuk</LastName><ForeName>Lawrence</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada (AAFC), Lethbridge, AB, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tremblay</LastName><ForeName>D Mathieu</ForeName><Initials>DM</Initials><AffiliationInfo><Affiliation>AAFC, St-Jean-sur-Richelieu, QC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carisse</LastName><ForeName>Odile</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>AAFC, St-Jean-sur-Richelieu, QC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Danies</LastName><ForeName>Giovanna</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fry</LastName><ForeName>William E</ForeName><Initials>WE</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lévesque</LastName><ForeName>C André</ForeName><Initials>CA</Initials><AffiliationInfo><Affiliation>AAFC, Ottawa, ON, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bilodeau</LastName><ForeName>Guillaume J</ForeName><Initials>GJ</Initials><AffiliationInfo><Affiliation>CFIA, Ottawa.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>1</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30686184</ArticleId><ArticleId IdType="doi">10.1094/PDIS-07-15-0763-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Ontario</li><li>État de New York</li></region><settlement><li>Ottawa</li></settlement></list><tree><country name="Canada"><noRegion><name sortKey="Gagnon, Marie Claude" sort="Gagnon, Marie Claude" uniqKey="Gagnon M" first="Marie-Claude" last="Gagnon">Marie-Claude Gagnon</name></noRegion><name sortKey="Bilodeau, Guillaume J" sort="Bilodeau, Guillaume J" uniqKey="Bilodeau G" first="Guillaume J" last="Bilodeau">Guillaume J. Bilodeau</name><name sortKey="Carisse, Odile" sort="Carisse, Odile" uniqKey="Carisse O" first="Odile" last="Carisse">Odile Carisse</name><name sortKey="Kawchuk, Lawrence" sort="Kawchuk, Lawrence" uniqKey="Kawchuk L" first="Lawrence" last="Kawchuk">Lawrence Kawchuk</name><name sortKey="Levesque, C Andre" sort="Levesque, C Andre" uniqKey="Levesque C" first="C André" last="Lévesque">C André Lévesque</name><name sortKey="Tremblay, D Mathieu" sort="Tremblay, D Mathieu" uniqKey="Tremblay D" first="D Mathieu" last="Tremblay">D Mathieu Tremblay</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Danies, Giovanna" sort="Danies, Giovanna" uniqKey="Danies G" first="Giovanna" last="Danies">Giovanna Danies</name></region><name sortKey="Fry, William E" sort="Fry, William E" uniqKey="Fry W" first="William E" last="Fry">William E. Fry</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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